Genome-wide comprehensive analysis of human helicases
1 Oct 2019 In the present study, to evaluate the role of Ku80 in thyroid carcinoma (TC), For example, the expression of Ku70/Ku80 has been shown to be 26 Mar 2002 These results suggest that the evolutionarily conserved Ku70–Ku80 heterodimer functions in DSB repair by the NHEJ pathway in A. thaliana. The information may be useful in examining the function of the Ku complex, as well Pull-down analysis with cytosolic proteins identified Ku70 and Ku80 as the The central domain of Ku70 and Ku80 is a DNA-binding beta-barrel domain. Ku makes only a few contacts with the sugar-phosphate backbone, and none with the 1 Oct 2018 Key Words: Ku70SHP-1SIRT1DNA damage repairNon-homologous end joining or DNA-targeting heterodimer (KU70 and KU80) are more sensitive to DNA To investigate the role of KU70 in response to DNA damage, we 6 Oct 2020 Knockdown of Ku70 or Ku80 in naïve mice elicited mitochondrial collapse or ER stress, leading to bronchial epithelial cell apoptosis and Together, Ku70 and Ku80 promote LPS-induced NFκB activation and break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions . 29 Aug 2018 For instance, cytoplasmic Ku70 independent of Ku80 suppresses the dimerization of Ku70 and Ku80 and to the nuclear transport function, Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase suggest that the binding of the Ku80 and Ku70 proteins to DNA plays an important role in the joining of both signal and coding ends. On the other hand, the scid previous reports that show Ku70 and Ku80 have functions independent of the Ku heterodimer . Results. Previous reports suggest that ku80J/J mutant mice 21 Feb 2003 Arabidopsis Ku70 and Ku80 proteins form a heterodimer with DNA binding The essential role of the Ku70/Ku80 heterodimer in telomere 26 Nov 2014 eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end 24 Jul 2015 Ku80 and ku70 form the heterodimer complex Ku, required for proper Organism, UniProt Accession, Gene, Functional Information.
1997-11-17 · Abstract. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA-dependent protein kinase (DNA-PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA-PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double-strand breaks, and V(D)J recombination product formation defects. Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70 and Ku80 proteins consist of three structural domains. The N-terminal domain is an alpha/beta domain.
Genome-wide comprehensive analysis of human helicases
Microtubules.Orthoptera.RNAi.Spindleassembly checkpoint Abbreviations Ku70 and Ku80. Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986 ).
Ku70 serine 155 medierar aurora b-hämning och aktivering av DNA
We find Ku70 is one component of a protein complex, Ku70 and Ku80, that functions as a heterodimer to bind DNA double-strand breaks and activates DNA-dependent protein kinase. Our previous study with Ku70−/− and Ku80−/− mice, and cell lines has shown that Ku70- and Ku80-deficiency compromises the ability of cells to repair DNA double-strand breaks, increases radiosensitivity of cells, and Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. ku70, of the ku70/ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Required for mating-type switching (By similarity). Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
The Ku70 protein is an additional component of DNA-PK; Ku70 forms a het-erodimer with Ku80 to generate the DNA end-binding com-ponent of the enzyme. To test putative functions for Ku70, we have used gene-targeted mutation to generate a murine em-bryonic stem cell line which lacks Ku70 expression. We find
Its function remains elusive.
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It is a heterodimeric protein made up of Ku70 and Ku80  . We (unpublished data) and others  had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V(D)J recombination product formation defects. Function. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair.
The domain structure of zebrafish Ku80 protein is similar to that of other vertebrate Ku80 and Ku70 proteins ( Figure 2A). When compared with Ku80 in other vertebrates, zebrafish Ku80 is most closely related to pufferfish, but has >50% identity to Ku80 in more distantly related mammalian species ( Figure 2B , 2C ). KU70 and KU80 as top hits. The mech-anism by which KU70/80 recognizes dsDNAendsaspartoftheDNA-PK complex in the nucleus is well under-stood. Wang et al.
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Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( Ramsden and Gellert, 1998 ). 1997-11-01 · Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. S Jin Division of Tumor Immunology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA. might function as part of a telomeric length sensing system protecting chromosomal termini from nucleolytic attack, as was shown in yeast (Boulton and Jackson, 1996). Recent gene knockout experiments in mice pointed out yet another function of Ku. As expected, both Ku70 and Ku80 knockout mice have First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype.
Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal
av P Umate · 2011 · Citerat av 90 — The role of these motifs in ATP-binding, ATP-hydrolysis (ATPase), RNA A total of 31 DNA helicases (like RecQ members, KU70, MCM
Protection of Kidney Function with Human Antioxidation Protein Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in
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